Prov Medium Derivatives
These media contain less Alkaline Soil Extract and are supplemented with NH4Cl. (To make 50 mM NH4Cl Stock Solution dissolve 2.68 g NH4Cl in 1.0 L dH2O).
Prepare as for Prov Medium, but use only 10.0 ml Alkaline Soil Extract. Add 1.0 ml 50 mM NH4Cl Stock Solution. Bring final volume up to 1.0 L with filtered seawater and autoclave after all additions.
Dispense Prov50 into desired culture vessel, add Organics Stock Solution then autoclave. Organics Stock Solution should comprise
5% of the final volume of this medium.
Organics Stock Solution:
Sodium Acetate 1.0 g
Glucose 6.0 g
Sodium Succinate 6H2O 3.0 g
Neopeptone 4.0 g
Bacto Tryptone 4.0 g
Yeast extract 2.0 g
Distilled water to 1.0 L
Dispense in small aliquots and autoclave.
Add one grain of white rice to a tube of Prov50 then autoclave. The addition of rice encourages bacterial growth, and is helpful when maintaining mixotrophic and heterotrophic strains.
Prepare as for Prov Medium, but use only 10.0 ml Alkaline Soil Extract. Add 2.0 ml 50 mM NH4Cl Stock Solution. Make final volume up to 1.0 L with filtered seawater and autoclave after all additions.
This medium is used to maintain organisms from brackish environments
(approximately 24 ppt salinity). Mix 750 ml filtered seawater and 200.0 ml
dH2O. Prepare as for Prov Medium, but add only 5.0 ml Alkaline Soil Extract. Make final volume up to 1.0 L with dH2O, then autoclave.
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