Antia's (ANT) Medium


KNO3 0.05 g
NaH2PO4 2H2O 0.0078 g
Tris 1.0 g
Glycine 0.3 g
Trace metals stock solution 2.5 ml
Thiamine HCl 500.0 g
Cyanocobalamin 2.0 g
Biotin 1.0 g
Filtered natural seawater 800.0 ml
Distilled water to 1.0 L

Autoclave at 15 psi. Final pH should be 7.6 - 7.8.

Trace metals stock solution:
EDTANa2 2H2O 3.24 g
FeCl3 4H2O 1.08 g
MnSO4 4H2O 0.450 g
ZnSO4 7H2O 0.230 g
Na2MoO4 2H2O 0.097 g
CuSO4 5H2O 0.01 g
CoSO4 7H2O 0.0056 g
Distilled water to 1.0 L

Adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen.



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