SN Medium

Mix 750 ml filtered seawater and 250 ml distilled H2O. Autoclave in a teflon lined bottle then add the following compounds aseptically:

NaNO3 (300.0 g/L dH2O) 2.5 ml
K2HPO4 (anhydrous) (6.1 g/L dH2O) 2.6 ml
Na2EDTA 2H2O (1.0 g/L dH2O) 5.6 ml
Na2CO3 (4.0 g/L dH2O) 2.6 ml
Vitamin B12 (1.0 mg/L dH2O) 1.0 ml
Cyano Trace Metal Solution 1.0 ml

Cyano Trace Metal Solution:
Citric Acid H2O 6.25 g
Ferric Ammonium Citrate 6.0 g
MnCl2 4H2O 1.4 g
Na2MoO4 2H2O 0.39 g
Co(NO3)2 6H2O 0.025 g
ZnSO4 7H2O 0.222 g

Dissolve each metal compound individually in 100 ml dH2O. Combine and mix the six solutions, then bring volume up to 1.0 L with dH2O.

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