Castenholtz ND Medium

Na2HPO4 0.11 g
MgSO4 7H2O 0.10 g
Nitrilotriacetic acid 0.10 g
CaSO4 2H2O 0.06 g
NaCl 8.0 mg
FeCl3 solution 1.0 ml
Castenholtz micronutrient solution 0.5 ml
Distilled water to 1.0 L

pH 7.5 +/- 0.2 at room temperature

Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.

FeCl3 solution:
FeCl3 6H2O 2.28 g
Distilled water to 1.0 L

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