Castenholtz D Medium

NaNO3 0.7 g
Na2HPO4 0.11 g
KNO3 0.10 g
MgSO4 7H2O 0.10 g
Nitrilotriacetic acid 0.10 g
CaSO4 2H2O 0.06 g
NaCl 8.0 mg
FeCl3 solution 1.0 ml
Castenholtz micronutrient solution 0.5 ml
Distilled water to 1.0 L

pH 7.5 +/- 0.2 at room temperature

Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.

FeCl3 solution:
FeCl3 6H2O 2.28 g
Distilled water to 1.0 L

Castenholtz micronutrient solution:
MnSO4 H2O 2.28 g
H3BO3 0.5 g
ZnSO4 7H2O 0.5 g
CoCl2 6H2O 0.025 g
CuSO4 5H2O 0.025 g
Na2MoO4 2H2O 0.025 g
H2SO4 0.5 ml
Distilled water to 1.0 L

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